After saponification, the lipid extract, called “unsaponifiables”, may contain other lipids besides sterols including hydrocarbons, carotenoids, tocopherols, free fatty acids, and other triterpenes. Many researchers proceed with derivatization and GC analysis without further sample cleanup and do not have problems with interference by these compounds. This is usually acceptable for the unsaponifiables fraction of a refined fat or oil sample. However, in some samples, such as crude oils, it may be necessary to further purify the sterol fraction from polar unsaponifiable lipids. Silica, C18, and aminopropyl SPE cartridges have all been used for this purpose. Phillips and co-workers  used aminopropyl SPE columns to separate sterols and stanols from serum unsaponifiable lipids, using chloroform: isopropanol for elution. Toivo and co-workers  demonstrated that equal yields were obtained using either C18 or silica SPE columns and eluting the sterol fraction from either column with 5% methanol in chloroform or 1% isopropanol in hexane, respectively.
I have often wondered why these X es appear directly above me. I must be a flyover, and that is why I like it indoors when the skies are hazy. I have not studied this matter in great detail, but I have no doubt that these are going to be used or have already been used for population control. Why else would you have to spin pharmaceuticals with nanoparticles and spin it into some cloud machine? I recall seeing a patent with some such strangeness. That might explain why all the homeless people across the USA have the same strain of Hep A. Or maybe it is from those vaccines they’re given, which are really not so safe if a person already has Hep C? That’s a mystery for another comment. I am guessing that when Fukushima extinction events occur, it is easier for them to spray people with the abortion pill than to deal with the negative publicity from malformed babies. I do not put anything past the atomic scientists or doctors employed by the government. They have always operated in their own little evil realm free from the law. I hope I am wrong, but nobody would tell me if I am right, so that is why you can get away with calling it a conspiracy theory. For now.
Total Bilirubin () is the sum of conjugated and unconjugated serum bilirubin. One of the byproducts of red blood cell breakdown is bilirubin. Bilirubin is made in the bone marrow, liver, or spleen and is transported by albumin to the liver in the form of unconjugated bilirubin. Once in the liver, sugars bind to the unconjugated bilirubin, turning it to conjugated bilirubin. It is then excreted from the gall bladder into the small intestine. Eventually, it is excreted in the feces or urine. Excess bilirubin causes a yellowing of the skin and the whites of the eyes called jaundice.